ANTIBACTERIAL EFFECTIVENESS TEST OF WRAP LEAF EXTRACT (Smilax Rotundifolia) AGAINST Escherichia Coli AND Propionibacterium Acnes BACTERIA

Diarrhea and acne, caused by the bacteria Escherichia coli andPropionibacterium acnes, are common illnesses in children and adults. Antibiotics canovercome bacterial infections, but cases of antibiotic resistance are increasing. Thisstudy aims to determine the effect of wrap leaf extract (Smilax rotundifolia) on thegrowth of P. acnes and E. coli. This research can support microbiology-based sciencelearning that utilizes biodiversity for students. Phytochemical tests showed that theextract contained flavonoids, alkaloids and saponins. The results of antibacterial testingshowed that wrap leaf extract was able to inhibit the growth of P. acnes bacteria fromconcentrations of 10% and 15% with an average inhibition of 5.31 mm and 6.42 mm,and the positive control (Amoxicillin) had an inhibition of 7.26 mm. However, E. colidid not show any inhibition zone. The Mann-Whitney test results showed that thewrapper leaf extract has antibacterial effectiveness by comparing the negative controlwith a concentration of 10% and 15% and the positive control with a concentration of10%. There is a difference between the concentration comparison where the Sig.<0.05value. Then, comparing the positive control with a concentration of 15% produces aSig.>0.05 value, meaning there is no difference between the two.